correlation of intracellular trehalose concentration with

correlation of intracellular trehalose concentration with

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results for this questionFeedbackCorrelation of intracellular trehalose concentration with

intracellular trehalose,proline,and glutamine under the desiccation condition than the hydrated control,and only the trehalose concentration exhibited a significant correlation with the desiccation-contributed die-off coefficients (Spearman's = -1.0; P = 0.02).De novo trehalose synthesis was further results for this questionHow does trehalose affect protein denaturation?How does trehalose affect protein denaturation?Trehalose inhibits protein denaturation by the exclusion of water molecules from the surface of proteins when cells are in the dehydrated condition 9.In the dry state,it maintains proteins in the folded state by replacing water molecules and forming hydrogen bonds directly with proteins 10.Mechanism of neuroprotection by trehalose controversy surrounding

results for this questionHow much Trehalose is in dormant cell extract?How much Trehalose is in dormant cell extract?According to 1 H- and 13 C-NMR spectroscopy up to 64% of total organic substances in the dormant cell extract was represented by trehalose whilst the trehalose content in an extract of active cells taken from early stationary phase was not more than 15%.Frontiers Free Trehalose Accumulation in Dormant results for this questionIs trehalose intracellular or extracellular?Is trehalose intracellular or extracellular?In mammalian cells,presence of intra- and extracellular trehalose has been shown to confer improved tolerance against freezing and desiccation.Since mammalian cells do not synthesize nor import trehalose,the development of novel methods for efficient intracellular delivery of trehalose has been an ongoing investigation.Engineered Trehalose Permeable to Mammalian Cells

(PDF) Correlation of Intracellular Trehalose Concentration

All E.coli strains produced more intracellular trehalose,proline,and glutamine under the desiccation condition than the hydrated control,and only the trehalose concentration exhibited a(PDF) High-resolution intracellular viscosity measurement If the change in intracellular mobility can be effectively measured as trehalose is loaded into the intracellular space,an experimental relationship can be established between the intracellular viscosity and intracellular trehalose concentration.This relationship is highly relevant in determining the nature of cellular protection,as methods

12345NextAuthor Changsheng Qiao,Shiru Jia,Yujie Dai,Rui Wang,Aiyou SunPublish Year 2010Osmometric behavior of mouse oocytes in the presence of

In the present study,murine (B6D2F1) oocytes were microinjected with 0.8M glucose,trehalose or stachyose solutions to achieve an intracellular concentration equivalent to 0.1M,and then exposed

Cited by 103Publish Year 2006Author Sooraj Ratnakumar,Alan TunnacliffeTrehalose Is a Key Determinant of the Quiescent Metabolic

Apr 28,2010 correlation of intracellular trehalose concentration with#0183;The treated medium was subsequently assayed for trehalose to ensure that it had been depleted of trehalose.SGal medium contained 6.7 g/l yeast nitrogen base without amino acids and 2% galactose.SGal plus trehalose medium contained 0.7 mg/ml trehalose (T9531; Sigma-Aldrich,St.Louis,MO) to mimic the concentration of trehalose present in YP Cited by 20Publish Year 2011Author Andrew L.Lynch,Nigel K.H.SlaterIntracellular trehalose is neither necessary nor Sep 01,2006 correlation of intracellular trehalose concentration with#0183;Conversely,however,other experiments suggest a negative correlation between intracellular trehalose concentration and desiccation tolerance cultures of mutant strains with a deleted TPS1 gene (tps1) that are unable to produce trehalose are nevertheless desiccation-tolerant and show improved survival of drying as they progress through the postdiauxic phase; heat stress

Cited by 56Publish Year 2012Author Qian Zhang,Tao Yan Correlation of Intracellular Trehalose Concentration

All E.coli strains produced more intracellular trehalose,proline,and glutamine under the desiccation condition than the hydrated control,and only the trehalose concentration exhibited a significant correlation with the desiccation-contributed die-off coefficients (Spearman's = 1.0; P = 0.02).Cited by 56Publish Year 2012Author Qian Zhang,Tao YanCorrelation of Intracellular Trehalose Concentration with Correlation of Intracellular Trehalose Concentration with Desiccation Resistance of Soil Escherichia coli Populations Qian Zhang and Tao Yan

Cited by 56Publish Year 2012Author Qian Zhang,Tao YanCorrelation of Intracellular Trehalose Concentration with

Oct 15,2012 correlation of intracellular trehalose concentration with#0183;The intracellular concentration differences between the desiccated condition and the hydrated control were calculated and correlated with their desiccation-caused die-off coefficients .Among the major intracellular organic solutes,trehalose,proline,glutamine,and glucose consistently showed elevated intracellular concentrations under the desiccated condition for all strains.Cited by 56Publish Year 2012Author Qian Zhang,Tao YanCorrelation of intracellular trehalose concentration with Correlation of intracellular trehalose concentration with desiccation resistance of soil Escherichia coli populations.Naturalized soil Escherichia coli populations need to resist common soil desiccation stress in order to inhabit soil environments.In this study,four representative soil E.coli strains and one lab strain,MG1655,were tested for desiccation resistance via die-off experiments in sterile quartz

Cited by 91Publish Year 1987Author M J McBride,J C EnsignMAL62 overexpression enhances uridine diphosphoglucose

Oct 19,2020 correlation of intracellular trehalose concentration with#0183;Moreover,in strains lacking trehalose synthesis,the cell viability in the late phase of prefermentation freezing coupled with MAL62 overexpression was slightly reduced,which can be explained by the increase in the intracellular glycerol concentration.This result was consistent with the elevated transcription of glycerol synthesis pathway Correlation of Intracellular Trehalose Concentration with FIG 5 Intracellular concentrations of trehalose in 10 soil E.coli strains (designated with an S),four E.coli isolates from municipal wastewater (designated by WW),and the lab strain MG1655 under the desiccation condition (solid bars) or the hydrated condition (open bars).- Correlation of Intracellular Trehalose Concentration with Desiccation Resistance of Soil Escherichia coli Populations

Correlation of Intracellular Trehalose Concentration with

Trehalose,proline,glutamine,acetate,valine,and glucose were identified as major organic solutes in the desiccated and hydrated E.coli cells by 1 H NMR.The intracellular concentration differences between the desiccated condition and the hydrated control were calculated and correlated with their desiccation-caused die-off coefficients (Table 2).Among the major intracellular organic solutes,trehalose,proline,glutamine,and glucose consistently showed elevated intracellular Correlation of intracellular trehalose concentration with All E.coli strains produced more intracellular trehalose,proline,and glutamine under the desiccation condition than the hydrated control,and only the trehalose concentration exhibited a significant correlation with the desiccation-contributed die-off coefficients (Spearman's =-1.0; P = 0.02).

Correlation of intracellular trehalose concentration with

Naturalized soil Escherichia coli populations need to resist common soil desiccation stress in order to inhabit soil environments.In this study,four representative soil E.coli strains and one lab strain,MG1655,were tested for desiccation resistance via die-off experiments in sterile quartz sand under a potassium acetate-induced desiccation condition.Correlation of intracellular trehalose concentration with desiccation resistance of soil Escherichia coli populations.Naturalized soil Escherichia coli populations need to resist common soil desiccation stress in order to inhabit soil environments.Author:Qian Zhang,Tao YanCited by:56Publish Year:2012Correlation of intracellular trehalose concentration with Was this helpful?People also askHow does trehalose affect the viability of a cell?How does trehalose affect the viability of a cell?Varying the concentration of free trehalose in dormant cells by expression of MSMEG_4535 coding for trehalase we found that cell viability depends on trehalose level cells with a high amount of trehalose survive much better than cells with a low amount.Frontiers Free Trehalose Accumulation in Dormant

Cryoprotective enhancing effect of very low concentration

correlation of intracellular trehalose concentration with#0183;Trehalose is a non-reducing disaccharide with two glucose molecules linked through an ,-1,1-glucosidic bond.Trehalose has received attention for the past few decades for its role in Effects Intracellular Trehalose Content StreptomycesThe relationship between glucose concentration in the mediumand trehalose content of spores was essentially linear between 5 and 100 mM glucose.Theamountsoftrehalose in spores harvestedfrom comparable liquid and solid media were similar (data not shown).Theeffect ofthe length ofincubation onagar sporulation mediumonthe trehalose

Engineering Saccharomyces cerevisiae for improvement in

Aug 02,2016 correlation of intracellular trehalose concentration with#0183;To measure the intracellular trehalose concentration during growth,the overnight culture was transferred into 100 mL of YPD broth to achieve an OD 620 value of 0.3 and incubated in 500 mL baffled Erlenmeyer Flasks at 30 correlation of intracellular trehalose concentration with#176;C with shaking (150 rpm) for 1,24,48,and 72 h.Free intracellular amino acid pools during autonomous Apr 20,2003 correlation of intracellular trehalose concentration with#0183;Accordingly,they exhibited different patterns in phase plane plots vs.intracellular trehalose.Despite the complex and marked metabolic changes during oscillation,selected intracellular amino acids such as histidine,threonine,isoleucine,or arginine remained about constant.Concentrations of glutamate and glutamine showed a chaotic behavior.

Frontiers Free Trehalose Accumulation in Dormant

Mar 30,2017 correlation of intracellular trehalose concentration with#0183;However,the intracellular concentration of trehalose in genetically modified dormant cells gradually decreased over the cell storage period and after 75 days was negligible (Figure 4).Estimation of viability of dormant cells by CFU and MPN assay revealed a direct correlation between trehalose content and cell viability (Figure 4).This Influence of intracellular trehalose concentration and pre Aug 01,2011 correlation of intracellular trehalose concentration with#0183;Cellular cryosurvival increased approximately linearly with pre-freeze cell volume up to the normal volume of fresh cells; diminished cell survival correlated with subnormal pre-freeze cell volume in some cases even at correlation of intracellular trehalose concentration withgt;100 mM intracellular trehalose concentration.

Intracellular osmoprotectant concentrations determine

Feb 19,2020 correlation of intracellular trehalose concentration with#0183;There is a correlation between the intracellular trehalose/GB ratio accumulated by P.freudenreichii and the lactose/NPN ratio of the growth medium composition (Fig.6(A)).The higher the lactose/NPN concentration ratio is,the higher the trehalose/NPN ratio will be.Investigating trehalose synthesis genes after cold Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp.DAW1 Anna C.Seybold1,David A.Wharton2,Michael A.S.Thorne3 and Craig J.Marshall1,4,* ABSTRACT Panagrolaimus sp.DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation.The molecular

Supporting Information - American Chemical Society

Linear correlation curve between the erythrocyte concentration counted using a haemocytometer and the haemoglobin absorbance at 541 nm after the lysis of those cells.Mean correlation of intracellular trehalose concentration with#177; S.D.(n = 3).Figure S4.Impact of PLP-NDA18 concentration on intracellular trehalose concentration and relative haemolysis.The involvement of trehalose in yeast stress tolerance The intracellular trehalose content of a number of yeast strains was monitored during growth on PYN media containing 5 70 glucose.As can be seen in Fig.1A with the osmotolerant Z.rouxii strain 233,the intracellular concentration of trehalose increased during growth reaching a maximum after 24-48 h.

Trehalose Biosynthesis Enhancement for Six Yeast Strains

Dec 03,2008 correlation of intracellular trehalose concentration with#0183;Six yeast strains of the commercial brewing yeasts CICC1391 and CICC1471,the commercial baker yeasts CICC1339 and CICC1447,and the commercial alcohol yeasts CICC1286 and CICC1291 have been cultured under 1.0 MPa of pressure with N2 and CO2 as pressure media.The concentration of intracellular trehalose and the activity of trehalose synthases complex have beenTrehalose promotes the survival of Saccharomyces Depending on the environmental conditions,trehalose can represent from correlation of intracellular trehalose concentration withlt;1% to correlation of intracellular trehalose concentration withgt;25% of cell dry weight (Hohmann,2002),and over the past 20 years,a number of studies have demonstrated a correlation between intracellular trehalose levels and the ability of yeast to survive various environmental stresses,such as starvation,desiccation

Zhang,Q.and Yan,T.(2012) Correlation of Intracellular

Zhang,Q.and Yan,T.(2012) Correlation of Intracellular Trehalose Concentration with Desiccation Resistance of Soil Escherichia coli Populations.Applied and Environmental Microbiology,78,7407-7413.


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